Author(s) Ressler, A.; Cvetnić, M.; Antunović, M.; Marijanović, I.; Ivanković, M.; Ivanković, H.
Journal J Biomed Mater Res B Appl Biomater
Date Published 2020 May

Biomimetic triphasic strontium-substituted calcium phosphate (CaP) powders were prepared by wet precipitation method at 50°C, using CaCO , (NH ) COH PO , and Sr(NO ) as reagents. Calcite was prepared from biogenic source (cuttlefish bone). The synthesized powders have been characterized by elemental analysis, Fourier transform infrared spectrometry, X-ray diffraction, Rietveld refinement studies and cell viability test. Phase transformation and ion release were analyzed during 7 days of incubation in simulated body fluid at 37°C. The raw precipitated powders were composed of calcium deficient carbonated hydroxyapatite (HA), octacalcium phosphate (OCP), and amorphous calcium phosphate (ACP). After heat treatment at 1200°C β-tricalcium phosphate (β-TCP) was detected. Strontium substitution for calcium results in an increase of lattice parameters in HA, OCP, and β-TCP. Sr occupy the Ca(1) site in HA, Ca(3,4,7,8) sites in OCP and Ca(1,2,3,4) sites in β-TCP. Along with Sr substitution, presence of Mg and Na ions was detected as a result of using biogenic calcium carbonate. The culture of human embryonic kidney cells indicated noncytotoxicity of the prepared CaP powders with emphasis on the cell proliferation during 3 days of culture.

DOI 10.1002/jbm.b.34515
ISSN 1552-4981
Citation J Biomed Mater Res Part B Appl Biomater. 2020;108(4):16971709.

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