Studying the roles of calcium and magnesium in cell death in the serpentine native plant Alyssum inflatum NYÁRÁDY through cell suspension culture technique.

Author(s) Ghasemi, R.; Sharifi, R.; Ghaderian, S.Majid
Journal Plant Physiol Biochem
Date Published 2020 Jun

Calcium is an essential element for plants' survival and ability to deal with environmental stresses. However, it can cause cell death due to cellular disequilibrium. Serpentine plants are sensitive to high concentrations of Ca, which induces lethal symptoms, especially under environmental stress. In this study, the direct effects of Ca on cell death were investigated in cell cultures of Alyssum inflatum, a serpentine plant native to Western Iran, and results were compared to a non-serpentinitic congeneric species A. saxatile. The results were also compared to the effects of Mg treatments in both species, as another determinative factor in serpentinite soil is high Mg content. Plasma membrane permeability, reactive oxygen species (ROS), and malondialdehyde (MDA) production were measured as physiological cell injury indices. In A. inflatum higher levels of ROS and MDA were observed in Ca-treated cells (5 mM or more), while in A. saxatile they were measured in Mg-treated cells (5 mM or more). In serpentine species, results indicated that cell death by Ca was more intensive than the cell death by Mg, which were observed with less intensity in non-serpentine plants. Microscopic studies showed that cell death occurred via apoptosis-like programmed cell death (AL-PCD). Therefore, Ca sensitivity and AL-PCD as mechanistic reasons for their non-serpentine intolerance would be a crucial consideration in cellular researches concerning serpentine plants, which could be employed in green technologies such as phytoremediation.

DOI 10.1016/j.plaphy.2020.03.032
ISSN 1873-2690
Citation Plant Physiol Biochem. 2020;151:362368.