Tetraphenylethene derivative modified DNA oligonucleotide for in situ potassium ion detection and imaging in living cells.

Author(s) Lu, D.; He, L.; Wang, Y.; Xiong, M.; Hu, M.; Liang, H.; Huan, S.; Zhang, X.B.; Tan, W.
Journal Talanta
Date Published 2017 May 15
Abstract

The monitoring of K(+) is very important and emergency because of their unique relationship in various disease diagnosis and treatment. G-quadruplex analogue is a classical recognition unit for K(+) detection and has been widely applied in K(+) relevant research. Common fluorescent dyes were employed for design of G-quadruplex structure-based K(+) probes which suffered from the aggregation-caused quenching effect, and possibly limited the biological applications in living systems. Herein, we report an aggregation-induced emission (AIE) effect-based fluorescent probe for cellular K(+) analysis and imaging. Benefitting from the K(+) triggered AIE phenomenon, the designed TPE derivative modified guanine (G)-rich oligonucleotide fluorescent probe (TPE-oligonucleotide probe) exhibits high sensitivity (∼10-fold higher than most reported G-quadruplex-based probes) with extended photostability which facilitates the prolonged fluorescence observations of K(+) in living cells. On the basis of these advantages, the TPE-oligonucleotide probe serves as a promising candidate for the functional study and analysis of K(+).

DOI 10.1016/j.talanta.2017.02.064
ISSN 1873-3573
Citation Lu D, He L, Wang Y, Xiong M, Hu M, Liang H, et al. Tetraphenylethene derivative modified DNA oligonucleotide for in situ potassium ion detection and imaging in living cells. Talanta. 2017;167:550-556.

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