Author(s) Ibrahim, A.A.; Ahmad, R.; Umar, A.; Al-Assiri, M.S.; Al-Salami, A.E.; Kumar, R.; Ansari, S.G.; Baskoutas, S.
Journal Biosens Bioelectron
Date Published 2017 Dec 15

Herein, we demonstrate synthesis and application of two-dimensional (2D) rectangular ytterbium oxide (YbO) nanodisks via a facile hydrothermal method. The structural, morphological, compositional, crystallinity, and phase properties of as-synthesized nanodisks were carried out using several analytical techniques that showed well defined 2D rectangular nanodisks/sheet like morphologies. The average thickness and edge length of the nanosheet structures were 20 ± 5nm and 600 ± 50nm, respectively. To develop urea biosensor, glassy carbon electrodes (GCE) were modified with YbO nanodisks, followed by urease immobilization and Nafion membrane covering (GCE/YbO/Urease/Nafion). The fabricated biosensor showed sensitivity of 124.84μAmMcm, wide linear range of 0.05-19mM, detection limit down to ~ 2μM, and fast response time of ~ 3s. The developed biosensor was also used for the urea detection in water samples through spike-recovery experiments, which illustrates satisfactory recoveries. In addition, the obtained desirable selectivity towards specific interfering species, long-term stability, reproducibility, and repeatability further confirm the potency of as-fabricated urea biosensor.

DOI 10.1016/j.bios.2017.06.015
ISSN 1873-4235
Citation Biosens Bioelectron. 2017;98:254260.

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