Vanadium pentoxide increased PTEN and decreased SHP1 expression in NK-92MI cells, affecting PI3K-AKT-mTOR and Ras-MAPK pathways.

Author(s) Gallardo-Vera, F.; Tapia-Rodriguez, M.; Diaz, D.; van der Goes, T.Fortoul; Montaño, L.F.; Rendón-Huerta, E.P.
Journal J Immunotoxicol
Date Published 2018 Dec
Abstract

Vanadium is an air pollutant that imparts immunosuppressive effects on NK cell immune responses, in part, by dysregulating interleukin (IL)-2/IL-2R-mediated JAK signaling pathways and inducing apoptosis. The aim of the present study was to evaluate effects of vanadium pentoxide (VO) on other IL-2 receptor-mediated signaling pathways, i.e. PI3K-AKT-mTOR and Ras-MAPK. Here, IL-2-independent NK-92MI cells were exposed to different VO doses for 24 h periods. Expression of PI3K, Akt, mTOR, ERK1/2, MEK1, PTEN, SHP1, BAD and phosphorylated forms, as well as caspases-3, -8, -9, BAX and BAK in/on the cells were then determined by flow cytometry. The results show that VO was cytotoxic to NK cells in a dose-related manner. Exposure increased BAD and pBAD expression and decreased that of BAK and BAX, but cell death was not related to caspase activation. At 400 µM VO, expression of PI3K-p85 regulatory subunit increased 20% and pPI3K 50%, while that of the non-pPI3K 110α catalytic subunit decreased by 20%. At 200 μM, VO showed significant decrease in non-pAkt expression (p < 0.05); the decrease in pAkt expression was significant at 100 μM. Non-pmTOR expression displayed a significant downward trend beginning at 100 μM. Expressions of pMEK-1/2 and pERK-1/2 increased substantially at 200 μM VO. No differences were found with non-phosphorylated ERK-1/2. PTEN expression increased significantly at 100 μM VO exposure whereas pPTEN decreased by 18% at 25 μM VO concentrations, but remained unchanged thereafter. Lastly, VO at all doses decreased SHP1 expression and increased expression of its phosphorylated form. These results indicated a toxic effect of VO on NK cells that was due in part to dysregulation of signaling pathways mediated by IL-2 via increased PTEN and decreased SHP1 expression. These results can help to explain some of the known deleterious effects of this particular form of vanadium on innate immune responses

DOI 10.1080/1547691X.2017.1404662
ISSN 1547-6901
Citation Gallardo-Vera F, Tapia-Rodriguez M, Diaz D, van der Goes TF, Montaño LF, Rendón-Huerta EP. Vanadium pentoxide increased PTEN and decreased SHP1 expression in NK-92MI cells, affecting PI3K-AKT-mTOR and Ras-MAPK pathways. J Immunotoxicol. 2018;15(1):1-11.

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